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American Society of Microbiology ABMM Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Directing Laboratory Testing Functions | 44.5% | - Up-to-date practices, standards, technologies and emerging diseases - Test selection, validation, quality control and interpretation |
| Topic 2: Ensuring Safety and Security in the Laboratory | 11.5% | - Security, waste management and emergency response - Biological, chemical and physical safety |
| Topic 3: Consulting with Other Medical Professionals | 25% | - Education, research and professional development - Clinical consultation, result interpretation and guidance - Public health, epidemiology and outbreak investigation |
| Topic 4: Directing Laboratory Administrative Functions | 19% | - Accreditation, licensure and regulatory requirements - Laboratory operations, budgeting and compliance - Personnel management, competency assessment and training - Information management and reporting - Process improvement and risk management |
American Society of Microbiology ABMM Sample Questions:
A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate recovered from a patient with meningitis. The isolate shows reduced susceptibility to penicillin (MIC = 2 µg/mL). The MOST likely mechanism for this reduced susceptibility is:
- A. Production of beta-lactamase enzymes that hydrolyze penicillin.
- B. Alterations in the penicillin-binding proteins (PBPs) that reduce their affinity for penicillin.
- C. Increased activity of efflux pumps that actively remove penicillin from the cell.
- D. Modification of the peptidoglycan structure, preventing penicillin binding.
Correct Answer: B 🗳️
A Gram stain of anaerobic growth from a peritoneal fluid abscess reveals pleomorphic, pale-staining Gram- negative rods. The isolate grows on Bacteroides Bile Esculin (BBE) agar, hydrolyzing esculin (producing black colonies), and is resistant to kanamycin (1000 µg), vancomycin (5 µg), and colistin (10 µg) disks. Which organism group is most likely?
- A. Prevotella melaninogenica group
- B. Bacteroides fragilis group
- C. Clostridium perfringens
- D. Fusobacterium nucleatum
Correct Answer: B 🗳️
A traveler returning from Southeast Asia presents with fever, headache, and a maculopapular rash that started on the trunk and spread to the extremities, including the palms and soles. Serological testing is positive for Orientia tsutsugamushi. The vector responsible for transmitting this organism is a:
- A. Mite (chigger)
- B. Flea
- C. Mosquito
- D. Tick
Correct Answer: A 🗳️
A clinical laboratory performs a cefoxitin disk diffusion test on a Staphylococcus aureus isolate from a wound culture. The zone of inhibition measures 18 mm. According to current CLSI M100 guidelines, how should this result be interpreted regarding oxacillin/methicillin susceptibility?
- A. Susceptible (report oxacillin as susceptible)
- B. Resistant (report oxacillin as resistant; infer MRSA)
- C. Intermediate (requires MIC testing)
- D. Indeterminate (requires mecA gene testing)
Correct Answer: B 🗳️
A clinical microbiology laboratory is using a nucleic acid amplification test (NAAT) to detect Mycobacterium tuberculosis directly from sputum. A positive result indicates:
- A. Drug susceptibility information for the M. tuberculosis strain.
- B. The presence of M. tuberculosis DNA, which could be from viable or non-viable organisms.
- C. Active tuberculosis disease requiring immediate treatment.
- D. The presence of viable M. tuberculosis organisms.
Correct Answer: B 🗳️
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